Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin
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Beschreibung
This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book.In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin.Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research.
This protocol book provides detailed procedures for the isolation of cyanobacteria, extraction, quantification, and detection of cyanotoxins. It illustrates the sampling and processing of toxin-producing cyanobacteria in water and aquatic animal samples, detection of cyanotoxins from Anabaena, Anabaenopsis, Cylindrospermopsis, Microsystis, Microcystis, Nodularia, Nostoc, Schizotrix, Lyngbya, Raphidiopsis, Oscillatoria, Planktothrix in aquatic resources. It also covers toxicity analysis by various bioassay protocols, and in vitro and insilico analysis methods. The book also reviews the methods for cyanotoxin extraction, detection, and quantification by various tools including LC-MS/MS, HPLC, NMR, PCR, and HESI-MS/MS. A separate section is dedicated to the advanced methods in Cyanotoxin analysis including the Molecular Imprinting Method (MIM), Cellular signaling biosensor, Electrochemical sensor, Nanosensors, and screening of Polyketide synthase gene. The analysis of various toxin-producing genes like sxtA and mcy is also accounted for in this book.
In a nutshell, the book gives comprehensive procedures about the basics and preliminary processes that are involved in sample collection to advanced methods incorporated into the well-explored and unexplored Cyanobacterial toxin.
Consequently, this manual is useful for both beginners and advanced researchers, including postgraduate students, academicians, researchers, and scientists in the field of Cyanobacterial research.
Illustrates isolation of Cyanobacteria, extraction, quantification & detection of Cyanotoxins from aquatic resources Includes protocols like Molecular Imprinting Polymer techniques, molecular detection of cyr C genes & CRISPR techniques Discusses procedure for the cyanobacterial toxin analysis
Autor*in
N. Thajuddin
Themen in »Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin«
Cyanotoxins Blue Green Algae Microcystins Aquatic animals Marine water