Nucleic Acid Detection
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Beschreibung
This book presents a comprehensive and up‑to‑date reference on nucleic acid detection technologies. It systematically introduces classical amplification‑based methods, including polymerase chain reaction (PCR), ligase chain reaction (LCR), loop‑mediated isothermal amplification (LAMP), rolling circle amplification (RCA), isothermal exponential amplification reaction (EXPAR), helicase‑dependent amplification (HDA), and recombinase polymerase amplification (RPA), as well as advanced nucleic acid detection approaches including CRISPR‑based technologies. In addition to methodological advances, the book provides an in‑depth overview of analytical strategies for key nucleic acid biomarkers. Bridging fundamental principles with application oriented insights, this volume serves as a valuable resource for researchers, graduate students, and professionals involved in nucleic acid testing, molecular diagnostics, and technology development across universities, research institutes, biotech companies, and clinical laboratories.
This book provides a comprehensive reference on nucleic acid analysis. It systematically introduces the molecular biology foundations, hybridization technologies, and advanced nucleic acid detection methods, including polymerase chain reactions, CRISPR-based systems, and high-throughput sequencing. Additionally, it explores the biological functions, analysis methods, and applications of various nucleic acid biomarkers, including DNA single nucleotide polymorphism, DNA methylation, microRNA, mRNA splice variants, fusion gene transcripts, RNA methylation and COVID-19 RNA. This book is valuable for researchers, postgraduates and practitioners engaged in nucleic acid analysis research and technological development in colleges and universities, scientific research institutes, nucleic acid testing-related companies and hospitals.
Covers molecular biology foundations and advanced nucleic acid analysis methods Explores biomarkers such as SNPs, DNA/RNA methylation, microRNA, and COVID-19 RNA with practical applications Provides systematic, comprehensive content with expert insights for research, diagnostics, and academic teaching
Autor*in
Zhengping Li
Themen in »Nucleic Acid Detection«
Nucleic acid analysis Biological functions of nucleic acids Biomarkers Biological chemistry