Super-resolution Imaging using Stochastic Optical Reconstruction Microscopy
Buch in deiner Nähe kaufen
Beschreibung
This book highlights super-resolution imaging using Stochastic Optical Reconstruction Microscopy (STORM). It begins with describing an optical microscope and its resolution limit due to the diffraction phenomenon. It also describes how diffraction barriers can be overcome by various optical super-resolution techniques. The book then discusses how super-resolution imaging can be achieved with the help of a simple experimental setup using the stochastic nature of fluorescence emission. It further elaborates the requirements in terms of excitation beam, fluorophore types, the imaging buffer and the sample preparation methods to observe fluorescence blinking, followed by a discussion on the image acquisition and the reconstruction algorithms. The book concludes with applications of STORM, its challenges and limitations.
Highlights optical super-resolution microscopy using STORM from theory to practical aspects of implantation Presents basics to advanced level of imaging, aiming for non-specialized readers and experts Addresses challenges and limitations in STORM microscopy, providing possible solutions and strategies
Autor*in
Bosanta Ranjan Boruah
Themen in »Super-resolution Imaging using Stochastic Optical Reconstruction Microscopy«
Diffraction limit Compound microscope Fluorescence microscope Point spread function Optical super-resolution Fluorophores Sustained blinking Photoswitching mechanism Image Acquisition and Reconstruction Photobleaching