Methods for Fragments Screening Using Surface Plasmon Resonance
Buch in deiner Nähe kaufen
Beschreibung
This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.
This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interactionof proteins using SPR.
Discusses real-time and label-free measurement of biomolecular interaction Present protocols to calculate kinetics and affinities with single analyte concentration Examines techniques for the optimum amine and biotin-streptavidin coupling Describes methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE)
Autor*in
Sameer Mahmood Zaheer
Themen in »Methods for Fragments Screening Using Surface Plasmon Resonance«
Surface plasmon resonance Protein stability Fragment library Thermal shift assay Amine coupling