Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions
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Beschreibung
This book provides essential insights into designing a localized DNA circuit to promote the rate of desired hybridization reactions over undesired leak reactions in the bulk solution. The area of dynamic DNA nanotechnology, or DNA circuits, holds great promise as a highly programmable toolbox that can be used in various applications, including molecular computing and biomolecular detection. However, a key bottleneck is the recurring issue of circuit leakage. The assembly of the localized circuit is dynamically driven by the recognition of biomolecules – a different approach from most methods, which are based on a static DNA origami assembly. The design guidelines for individual reaction modules presented here, which focus on minimizing circuit leakage, are established through NUPACK simulation and tested experimentally – which will be useful for researchers interested in adapting the concepts for other contexts. In the closing section, the design concepts are successfully applied to the biomolecular sensing of a broad range of targets including the single nucleotide mutations, proteins, and cell surface receptors.
Offers readers essential insights into designing a localized DNA circuit to promote the rate of desired reaction over undesired leak reactions in the bulk solution Proposes design concepts for individual reaction modules, with a focus on minimizing circuit leakage Covers applications for the biomolecular sensing of a broad range of targets including the mutation of nucleic acids, protein, and cell surface receptors
Autor*in
Ang Yan Shan
Themen in »Engineering a Robust DNA Circuit for the Direct Detection of Biomolecular Interactions«
DNA Computing Dynamic DNA Nanotechnology DNA Nanodevices Nupack Simulation Biomolecular Sensing Nuclei Acids Mutation Protein Surface Receptors Molecular Computing DNA Origami Assembly DNA Split Proximity Circuit biochemical engineering systems biology