Fundamentals of Fluorescence Microscopy
Exploring Life with Light
Buch in deiner Nähe kaufen
Beschreibung
This book starts at an introductory level and leads reader to the most advanced developments in fluorescence imaging and super-resolution techniques that have enabled the emergence of new disciplines such as nanobioimaging, multiphoton microscopy, photodynamic therapy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going deep into the advanced imaging systems and their applications. Molecular orbital theory forms the basis for understanding fluorescent molecules and thereby facilitates complete explanation of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential fluorescence factors and phenomena such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These phenomena form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part covers many imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction limited and unlimited imaging.
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.
The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
A comprehensive text that starts from the fundamental optical imaging concepts Guides the readers to the most advanced methods in fluorescence imaging and super-resolution techniques An essential text for courses in imaging optics, photophysics, fluorescence microscopy and imaging, methods in cell biology A thorough introduction to fluorescence microscopy and imaging for students in biophysics, molecular and cell biology, biomedical engineering, applied physics Contains three useful Appendices Lists of recommended books at the end of each chapter, to expand on various specialized topics Written by two acknowledged experts in this rapidly evolving, interdisciplinary field Includes supplementary material: sn.pub/extras
Autor*in
Partha Pratim Mondal
Themen in »Fundamentals of Fluorescence Microscopy«
Advanced fluorescence microscopy techniques Advances in fluorescence spectroscopy and microscopy Fluorophores and fluorescent probes Fourier, Fresnel, Fraunhofer geometrical optics Imaging optics Molecular physics and photophysics Quantitative fluorescence microscopy Ray optics and wave optics Super-resolution microscopy Textbook on fluorescence imaging Biological Microscopy
Stimmen zu »Fundamentals of Fluorescence Microscopy«
“Mondal and Diaspro have produced an introductory text providing all the necessary scientific background for students in applied physics, biophysics and biomedical engineering, Their text is also intended to be used by scientists and engineers in both industry and academia. … The book is generally well written for the intended readership … . It is nicely produced with large (almost A4 sized) glossy pages and some very attractive and interesting coloured images.” (Peter V. E. McClintock, Contemporary Physics, Vol. 58 (2), December, 2016)
()