Solid State NMR Spectroscopy for Biopolymers
Principles and Applications
Buch in deiner Nähe kaufen
Beschreibung
Solid State NMR Spectroscopy for Biopolymers
Principles and Applications
by Hazime Saitô, Isao Ando and Akira Naito
Unique and comprehensive coverage of solid state NMR, emphasising
secondary structure and dynamics in relation to biological function.
When considering the biological significance and industrial and medical applications of biopolymers, it is crucial to know details of their secondary structure, dynamics and assembly. The biopolymers include globular, membrane and fibrous proteins, polypeptides, nucleic acids, polysaccharides and lipids. Solid state NMR spectroscopy has proved to be the most suitable and unrivaled means for investigations of biopolymers. The major advantage of solid state NMR spectroscopy is that the resulting line widths can be manipulated experimentally and are not influenced by motional fluctuation of proteins under consideration as a whole.
Solid State NMR Spectroscopy for Biopolymers: Principles and Applications provides a comprehensive account on how the conformation and dynamics of such biopolymers can be revealed by solid state NMR spectroscopy. Special efforts have been made towards the historical and chronological consequences of a variety of applications and the dynamic aspects of the biopolymer system. In particular, the authors emphasise how important it is to record the most simple DD-MAS (one pulse excitation with high power decoupling) as a mean of locating very flexible portions of membrane proteins and membrane associated peptides. The authors also demonstrate that dynamic features of membrane proteins with a timescale of fast (108 Hz) and intermediate (104 -105 Hz) fluctuation motions can be revealed easily by specific suppression of peaks.
This book is an invaluable resource for biophysicists, biochemists and chemists, including NMR spectroscopists, structural biologists, and polymerscientists. The book provides an introduction suitable for graduate students as well as for research scientists, including those working in the pharmaceutical and chemical industries.
When considering the biological significance and industrial and medical applications of biopolymers, it is crucial to know details of their secondary structure, dynamics and assembly. Solid state NMR spectroscopy has proved to be the most suitable and unrivaled means for investigations of biopolymers. Special efforts have been made to include the historical and chronological consequences of a variety of applications and the dynamic aspects of the biopolymer system. In particular, the authors emphasise how important it is to record the simplest DD-MAS as a mean of locating very flexible portions of membrane proteins and membrane associated peptides. The authors also demonstrate that dynamic features of membrane proteins with a timescale of fast and intermediate fluctuation motions can be revealed easily by specific suppression of peaks.
Uniquely presents a comprehensive account of solid state NMR with an emphasis on revealing secondary structure and dynamics in relation to biological functions. Biopolymers-oriented, not just methodology-oriented. Not a compilation of recent progress on solid state NMR, as often attempted by other authors. Focuses on characterization, revealing secondary structure and dynamics as revealed by solid-state NMR. Emphasis on the chronological account of such studies.
Autor*in
Hazime Saitô
Themen in »Solid State NMR Spectroscopy for Biopolymers«
3D structure Biopolymer Dynamics Membrane proteins Membrane-associated peptides Solid State NMR crystal liquid nuclear magnetic resonance (NMR) polymer spectroscopy
Stimmen zu »Solid State NMR Spectroscopy for Biopolymers«
From the reviews:
"This book surveys much of the current research in the area of biological solid-state NMR spectroscopy, and as such should be of great interest to the chemical, biochemical, and biophysical communities. The level is appropriate for graduate students, and the book would be an excellent textbook for a graduate level course in biological solid-state NMR spectroscopy. Its publication is timely considering the recent numerous developments in this area … ." (Michele Auger, Journal of the American Chemical Society, Vol. 129 (10), 2007)
()