Validierung und Evaluierung einer Alternativen Probenentnahmetechnik zur Corticosteronbestimmung in Federn
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Beschreibung
"Validation and Evaluation of an Alternative Sampling Technique for Corticosterone Measurements in Feathers"
The aim of this work is to validate an alternative, non-painful and therefore less-invasive feather sampling method. In the future, feathers collected for subsequent CORTf analysis can be cut near the skin of living birds instead of plucking them. The background of this study is a science and animal based investigation on the welfare of birds under human care with different flight status. In this regard, the study design is built by a combination of measurement and interpretation of CORTf and behavioral observations.
The previous standard sampling method for CORTf measurements is feather plucking, which is considered as an animal experiment in Germany and other European countries. This work acts in the sense of animal welfare to reduce the number of animal experiments and refinement of procedures. To confirm the hypothesis that the sampling method itself has no effect on measured CORTf levels, both plucked and cut feathers were collected from each bird being examined. A total of two bird species from a conventional free-range poultry farming, which had the same living conditions, and two wild bird species from different habitats were studied. The former group consisted of domestic geese (Anser anser domseticus) and Muscovy ducks (Anas sterilis or Cairina moschata domestica x Anas platyrhynchos domesticus), a hybrid of Muscovy duck (Cairina moschata domestica) and Peking duck (Anas platyrhynchos domesticus). The second group were represented by a population of Greater Flamingos (Phoenicopterus roseus) from Spain and Mallards (Anas platyrhynchos) from Germany.
CORTf levels were measured using an ELISA. The values obtained have been compared for their mean, standard deviation, and average differences. Subsequently, the CORTf values were analyzed with the concordance correlation coefficient (CCC) depending on their sampling method. A comparison of the differences of the two methods with their respective mean values took place with the creation of the Bland-Altmann plot. Sex as a possible influencing factor to CORTf was examined using the Mann-Whitney U test in Greater Flamingos and Mallards. The CCC value did not show good agreement, whereas its graph, the Pearson's correlation coefficient, and the Bland-Altmann plot were acceptable. The average differences between the methods were marginal for all four species. In summary, any deviations, or differences between the two sampling techniques were negligible. Therefore, the alternative, less invasive sampling method appears to be as suitable as the previous standard method. No significant difference between females and males was found. Nevertheless, the results illustrate that a single interpretation of CORTf values is not useful, they should rather be analyzed in combination with other parameters, such as biological data and behavioral observations. Therefore, further studies are recommended to create a larger pool of data.
Autor*in
Marielu Voit
Themen in »Validierung und Evaluierung einer Alternativen Probenentnahmetechnik zur Corticosteronbestimmung in Federn«
Enten Federn Gänse Kortikosteron Tierschutz Wildvögel Zoologische Gärten animal welfare corticosterone ducks feathers geese phoenicopteridae wild birds zoological gardens