Evaluation of process designs for heterologous rhamnolipid production by Pseudomonas putida KT2440

Beschreibung

This doctoral thesis focuses on improving the upstream process of the rhamnolipid production. The main purpose was to increase biomass endurance, to raise final rhamnolipid titer and to avoid the application of substances complicating downstream processing. To reach these goals, different process designs were developed. In these process designs foam fractionation cultivations, model approaches and stirring as foam disruption were investigated and evaluated. Even with further optimization possible, this work showed up process designs for effective rhamnolipid production. Results showed higher rhamnolipid concentrations, avoidance of antifoaming agents and longer duration of biomass utilization. These properties are important prerequisites for the road to sustainable and cost effective biosurfactant production.

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Janina Beuker

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Rhamnolipid Pseudomonas putida Foam

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