Genotoxic Impacts on Marine Ecosystems
Buch in deiner Nähe kaufen
Beschreibung
Purpose of this review is to delineate the possibilty to adapt
the method of alkaline DNA unwinding for large scale routine
assessments of genotoxic impacts on marine ecosystems.
Purpose of this review is to delineate the possibilty to adapt
the method of alkaline DNA unwinding for large scale routine
assessments of genotoxic impacts on marine ecosystems.
DNA damage is a key-event in genotoxicity, which is conside-
red to increase risks for mutagenesis, carcinogenesis and va-
rious other - possibly even more population and ecosystem
relevant - toxicological end-points [Herbert & Hansen 1992,
Kurelec 1993]. Many links between the molecular event, the
primary DNA lesion, and possible population and ecosystem
effects - mediated through changes in viability and fertility with
species specificity - are, however, not yet fully understood and
still hypothetical. Part of this problem is, that methods suitable
for large-scale studies on pollution related DNA damage in the
environment have become available only recently and there-
fore data concerning the matter are still rare.
A DNA unwinding/HAP batch elution assay - initially develo-
ped to study DNA damage in mammalian cell cultures [Kanter
& Schwartz 1979] - has been adapted to study DNA damage
in tissues and blood or hemolymph samples of aquatic ani-
mals [Herbert 1987, Herbert & Zahn 1989, 1990, Herbert
1990, Herbert & Hansen 1992]. This work was aimed to provi-
de a tool for large-scale DNA damage monitoring in marine
environmental research, which meets the following require-
ments:
• wide spectrum of sensitivities providing a sum parame-
ter for different types of DNA lesions
• high cost efficiency
• easy use
rapidity (results in less than 1 day)
• high sample throughput (40 to 80 samples per investiga-
tor per day)
• portability for the use in field studies, e.g. on research
vessels and field stations
• no demand for radioactively labelled or highly toxic che-
micals
The investigations summarized in this review indicate suitabili-
ty of the chosen method in the study of marine fish, marine
fish embryos and marine invertebrates.
Autor*in
Armin Herbert
Studium der Biologie in Giessen, Tübingen und Mainz, Forschungstätigkeit in Meeresökologie und Umweltschutz am Meeresinstitut des Rudjer-Boskovic-Instituts In Rovinj (Jugoslawien, jetzt Kroatien), an der University of Florida (USA), der Freien Universität Berlin und Technischen Universität Berlin. Stipendiat der Deutschen Forschungsgemeinschaft.
Themen in »Genotoxic Impacts on Marine Ecosystems«
Meeresforschung Umweltschutz Erbgutveränderungen Krebserkrankungen Ökologie Ökotoxikologie Meereökologie