Microinjection and Transgenesis
Strategies and Protocols
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Beschreibung
The technique of microinjection is used to tranfer biological materials - RNA, proteins, oligonucleotides and especially cloned genes - into cultured somatic cells or embryos. Transgenic cells or animals - mice, rabbits or insects - allow the study of a wide range of cellular processes, such as gene expression, signal transduction or the cytoskeleton. More than 30 protocols on microinjection experiments and transgenesis studies developed in renowned laboratories are described in this manual. Special topics are the generation of transgenic mice using YAC, ligand-dependent site-specific recombination in embryonic stem cells, morula aggregation to generate germline chimeras as well as the analysis of injected cells.
The most potent technique to study cell function is the specific tranfer of genetic material into a single cell. Several functions can than be analyzed in the resulting transgenic cell or organism. The method of microinjection and transgenesis is currently used in many cell biological laboratories.
A practical tool presenting the latest techniques developed in renowned laboratories Easy to follow step-by-step protocols Background information and full practical details (materials needed, exact recipes, troubleshooting guide, typical results, list of suppliers, etc.)
Autor*in
Angel Cid-Arregui
Themen in »Microinjection and Transgenesis«
Vivo biochemistry cell cell culture chromosome electron microscopy gene gene expression gene transfer microscopy proteins ribozyme stem cells tissue transcription