Basic Cloning Procedures
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Beschreibung
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
Basic laboratory techniques, approved in several international molecular biology courses Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information The protocols are written by authors with intensive practical experience
Autor*in
Valdis Berzins
Themen in »Basic Cloning Procedures«
DNA RNA gene nucleic acid protein protein synthesis sequencing synthesis