Nucleic Acid Electrophoresis
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Beschreibung
Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.
Electrophoresis is a powerful method to analyze nucleic acids (DNA, RNA). Various sophisticated techniques such as capillary electrophoresis, pulsed-field electrophoresis, fingerprinting using RFLP and RAPD, DNA sequencing, and mobility shift assay are described in detail. The required apparatus, appropriate use, preparation of probes, gel staining, interpretation of results, tricks for troubleshooting, manufacturers addresses, helpful Internet resources as well as specific applications, e.g. in legal medicine, microbiology and agriculture, are presented by leading experts.
Step-by-step protocols with materials, troubleshooting and suppliers sections for immediate use at the lab bench All technical details needed to perform successful experiments are included Clear structure and presentation Protocols are written by experts in the relevant techniques
Autor*in
Dietmar Tietz
Themen in »Nucleic Acid Electrophoresis«
DNA DNA sequencing Elektrophorese Forensische Medizin Pathogen Polyacrylamid-Gelelektrophorese Protein-DNA Interaktionen RNA analytical biochemistry biochemistry chemistry forensic sciences microbiology preparation protein