Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods
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Beschreibung
Proteomics, a branch of functional genomics, can be defined as the large-scale analysis of proteins. In all these large-scale analysis processes there are three phases: (1) separation of the molecules to be analyzed; (2) characterization of the separated molecules; and (3) managing, handling and analysis of the data. This book is centered on the first two phases of proteomics, i.e. separation by two-dimensional electrophoresis and microcharacterization of the separated proteins. This choice is due to the fact that the data production phase is the major bottleneck of proteomics, while it is not the case in standard genomics. Emphasis has been placed on the rationale of each technique, so that the reader will be able to keep pace with the rapid evolution of the techniques. However, up-to-date protocols are also given to help the beginner in the field.
Two-dimensional electrophoresis is the central methodology in proteome research, and the state of the art is described in detail in this text, together with extensive coverage of the detection methods available. Sufficient detail is given to allow the readers to apply these technologies to their own particular requirements.
This is the first book to comprehensively cover the application of 2D gel technology to the field of proteomics Includes supplementary material: sn.pub/extras
Autor*in
T. Rabilloud
Themen in »Proteome Research: Two-Dimensional Gel Electrophoresis and Identification Methods«
2D Elektrophorese 2D electrophoresis Aminosäure-Sequenzierung Protein-Massenspektrometrie Protein-Nachweis Protein-Sequenzierung Proteome amino acid sequencing protein dectection protein mass spectrometry amino acid chemistry Elektrophorese genes genome