Gigaseal Formation in Patch Clamping
With Applications of Nanotechnology
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Beschreibung
This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.
Explains the mechanisms of gigaseal formation to the latest level of discoveries Provides practical techniques, which enable frequent formation of high resistance seals Shares authors´ recent practice in acquiring gigaseal formation using micropipettes Includes supplementary material: sn.pub/extras
Autor*in
Majid Malboubi
Themen in »Gigaseal Formation in Patch Clamping«
Gigaseal Formation using micropipettes High Resistance Seals High throughput screening Hydrophilicity Nanotechnology Patch Clamp Patch clamping Surface Roughness Tip Size
Stimmen zu »Gigaseal Formation in Patch Clamping«
“This is a very good book on patch clamp protocol with a focus on the engineering and physics of pipettes. A description on tuning for bore diameter and various approaches to the culture dishes is detailed. … This is a reliable resource for use in the physiology labs. Physiologists, students, postdocs, & senior investigators will find this very helpful.” (Joseph J. Grenier, Amazon.com, September, 2015)
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