Measuring Calcium and Calmodulin Inside and Outside Cells
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Beschreibung
The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signalling molecules as well as very many pathophysiological processes including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles and in the immediate surroundings of individual cells are described in practical details by experts, on the basis of their personal experience. Considering that the protein calmodulin is the main target for Ca2+ action in most cells types, a chapter on tracking calmodulin inside cells, using fluorescence technology, has also been included.
The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signaling molecules as well as many pathophysiological processes, including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles, and in the immediate surroundings of individual cells are described in practical detail by experts. Also included is a chapter on tracking calmodulin inside cells using fluorescence technology. A Springer Lab Manual
Step by step protocols of the various techniques for measuring Ca2+ inside and outside cells and organelles
Protocols contain the experience of researchers who did pioneering work in the field of measuring Ca2+ and tracking calmodulin inside cells
Autor*in
Ole H. Petersen
Themen in »Measuring Calcium and Calmodulin Inside and Outside Cells«
Calcium Fluorescence measurements calcium distribution calmodulin cancer cell cells electrophysiological measurements endoplasmic reticulum intracellular messenger mitochondria neurotransmitter protein signal transduction