Genetic Library Construction and Screening
Advanced Techniques and Applications
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Beschreibung
Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.
A comprehensive, up-to-date guide on all aspects of synthesis and screening of genetic libraries
Detailed step-by-step protocols and extensive hands-on advice gleaned from the authors' experience
Perfectly suited for experienced researchers and graduate students using these technologies in the lab
Autor*in
R.C. Bird
Themen in »Genetic Library Construction and Screening«
2-Hybrid DNA Gene Cloning Gene Library Molecular Biology PCR Polymerase Chain Reaction RNA cDNA cloning genome hybridization