Basic Techniques in Molecular Biology

Beschreibung

This laboratory manual gives a thorough introduction to modern, cost efficient techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses and workshops or tested in the author's laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed.
Step-by-step protocols and practical notes are provided. The distinguishing feature of this manual compared to others is the detailed explanation of the theoretical mechanisms of each step and the discussion of the importance of the techniques. In addition, each protocol includes an indication of the time and expense involved in its application. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.
Dr. Surzycki has been teaching undergraduate courses and leading workshops in introductory Molecular Biology for many years, during which time he has extensively modified and refined the techniques he has described in this manual.
This laboratory manual gives a thorough introduction to basic techniques in Molecular Biology, which can find application in many different fields. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. Thus, they are also likely to work in inexperienced hands the first time they are performed. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This knowledge will enable the users to design their own modifications or to adapt the method to different systems.
Surzycki has been teaching undergraduate courses and leading workshops for many years, during which time he has extensively modified and refined the techniques he has described in this manual.
Laboratory manual with detailed step-by-step protocols All of the procedures have either been used extensively in the teaching of undergraduate laboratories and adult workshops or tested in the authors laboratory Each technique includes an overview of its importance and theoretical background An ideal introduction to molecular biology techniques Includes supplementary material: sn.pub/extras

Autor*in

Stefan Surzycki

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DNA DNA Isolierung DNA sequencing Nucleic acids Nukleinsäuren PCR Polymerasekettenreaktion RNA Seuqenzierung Southern Blot biology cloning hybridization molecular biology preparation

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