Basic Cloning Procedures
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Beschreibung
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.
Basic laboratory techniques, approved in several international molecular biology courses
Step-by-step protocols with detailed practical hints, troubleshooting guide and short background information
The protocols are written by authors with intensive practical experience
This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
Autor*in
Valdis Berzins
Themen in »Basic Cloning Procedures«
DNA RNA gene nucleic acid protein protein synthesis sequencing synthesis