Signal Transduction — Single Cell Techniques
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Beschreibung
A variety of powerful techniques to monitor and analyse the events during signal transduction at the single cell level are described in this lab manual. An introductionary section for cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.
A variety of powerful techniques for monitoring and analysing events during signal transduction at the single cell level are described in this lab manual. An introductionary section on cell handling includes guidelines for constructing a perfusion chamber. A main section of the book presents protocols on fluorescence techniques such as flow cytometry, microfluorescence, ion imaging and confocal microscopy. The electrophysiological section illustrates multiple applications of the patch-clamp technique in various cell types from both animals and plants. Emphasis is put on calibration and validation of the different techniques to measure changes of membrane potential, and intracellular ion concentration or pH.
Labormanual mit aktuellem Überblick zur Zellkommunikation, einem der interessantesten Forschungsthemen der Zell- und Molekularbiologie
Ausführliche Darstellung verschiedener Techniken und Protokolle, mit denen die Veränderungen während der Signalübertragung an einer einzelnen Zelle verfolgt werden können
Cell cummunication is currently one of the most interesting topics in cell and molecular biology research. Various techniques to record the events and changes during signal transduction at the single cell level are described in this lab manual.
Autor*in
Bert, van Duijn
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