Plant Molecular Biology — A Laboratory Manual

Beschreibung

This manual covers the whole range of molecular biology techniques, genetic engineering as well as cytogenetics of plants. Each chapter starts with an introduction into the basic approach followed by detailed methods sections with easy-to-follow protocols and comprehensive troubleshooting. The first part of the book introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning. The second part describes analytical approaches, in particular RAPD and RFLP, while the final part encompasses a variety of gene transfer techniques and both molecular and cytological analysis. The manual will be of great use to both the first-timer and the experienced scientist.
The aim of this manual is to encompass a broad range of the latest plant molecular biology techniques. However, it is acknowledged that any manual will be read (and hopefully) used by a wide range of people with different levels of experience. Hence the remit of the manual was widened to include a full range of basic molecular tech niques, so that novices do not have to consult several texts to enable the execution of each major experiment. The manual is divided into three main parts: Part I: Basic Molecular Techniques The raison d'etre behind this part is to provide a background knowledge of molecular techniques, but also to reduce duplication in later chapters (this is particularly true of the methods contained in Chap. 1). All authors provided very detailed methods and often forgot that so me of these would be covered earlier. A particular favourite was DNA extraction methods, wherc everyone managed to provide a slightly different variant! My view was that it is far less confusing for the reader to be presented with one standard protocol and accom panying troubleshooting tips, than to read a different version in each chapter. In this way the basic techniques are addressed more in depth (and my apologies to all authors for judicious use of the delete key!). RNA methodology is covered in Chapter 3. This proceeds from the fundamentals of extraction, northern blotting etc. , to cDNA libraries.
This manual covers the whole range from basic molecular techniques through genetic engineering and a variety of specific protocols including cytogenetics. With detailed methodological sections and comprehensive troubleshooting it will be of great use to both the beginner as well as the experienced scientist.

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Melody S. Clark

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DNA PCR RNA YAC biology cytogenetics gene gene transfer genes genetic engineering genetics molecular biology plant plant genetics plants

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"Overall, this manual deserves to be highly recommended due, in particular, to the clarity of writing. ...this manual will be purchased and used by many in this rapidly expanding area of research." Heredity
"...presents a broad range of techniques needed by plant molecular biologists. The protocols are clearly presented and referenced." The New Phytologist
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