In Situ Hybridisation

Beschreibung

Since the first description of fluorescence in situ hybridisation (FISH) some ten years ago, this method has been used widely in laboratories and research areas, especially in cytogenetics. FISH, like related hybridisation techniques, is a very powerful method that has been applied to nearly every type of tissue, including human biopsy and autopsy samples, insects embedded in amber, mouse embryos, plant cell cultures, and naked dilated DNA, mRNA or chromosomes. Furthermore, it can be used both at the light and electron microscopic level. This bench-top manual includes easy-to-follow protocols which can be regarded as a basis for many specific experiments.

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Gary Coulton

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