Probes and Tags to Study Biomolecular Function
for Proteins, RNA, and Membranes
Buch in deiner Nähe kaufen
Beschreibung
In order to study living cells and their component molecules, two basic strategies have emerged in recent years. The first is to apply a soluble marker molecule, a 'probe', that partitions to a particular cellular domain, and which can subsequently be measured. The second is to chemically or genetically modify a molecule of interest with a built-in probe, which is then called a 'tag'.
This handy reference systematically reviews current experimental methods and enables researchers to select the best solution for their experimental problems. For each method covered, the book provides step-by-step protocols, illustrated by typical research applications. Commencing with probing the lipid bilayer, the text then moves on to discuss probing proteins -- including membrane proteins -- and nucleic acids. This is the first single publication to incorporate chemical markers, fluorescent probes and genetic tags to allow a well-informed comparison of different solutions for similar problems in molecular analytics.
Nach einer kurzen allgemeinen Einführung in die Untersuchung der Lipiddoppelschicht stellt Ihnen dieses langerwartete Handbuch systematisch alle modernen Methoden zur Analyse von Proteinen, Membranproteinen und Nukleinsäuren vor (chemische, genetische und Fluoreszenzmarker). Ausführliche Schritt-für-Schritt-Protokolle, ergänzt durch typische Anwendungsbeispiele, erleichtern Ihnen die Auswahl des geeigneten Ansatzes zur Lösung Ihres speziellen analytischen Problems.
Autor*in
Lawrence W. Miller
Themen in »Probes and Tags to Study Biomolecular Function«
Biomembran Biomolecules (DNA, RNA, Peptides, etc.) Biomoleküle (DNA, RNA, Peptide) Biowissenschaften Chemie Chemistry Life Sciences Membran Molecular Biology Molekularbiologie Nanobiotechnologie Nanobiotechnology Nanotechnologie Nanotechnology Pharmaceutical & Medicinal Chemistry