Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging
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Beschreibung
This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of abimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model. This is the first book to demonstrate the potential of SRDAs for fluorescence imaging in biomedicine, opening up a new multidisciplinary research direction.
This book describes the design, development, characterisation and application of two novel fluorescence imaging instruments based on spectrally resolved detector arrays (SRDAs). The simplest SRDA is the standard colour camera, which integrates a Bayer filter array of red, green and blue colour filters to replicate the colour sensing capability of the human eye. The SRDAs used in this book contain many more colours, ranging from 16 to over 100 colour channels. Using these compact, robust and low-cost detectors for biomedical applications opens new avenues of exploration that were not possible before, in particular, the use of spectral imaging in endoscopy. The work presented shows for the first time that not only can this new type of camera be used for fluorescence imaging, but also that it is able to resolve signals from up to 7 different dyes – a level of multiplexing not previously achieved in tissue with such compact and robust equipment. Furthermore, it reports the application of abimodal endoscope performing both reflectance and fluorescence imaging using these cameras in an ex vivo pig oesophagus model.
Nominated as an outstanding Ph.D thesis by the University of Cambridge, Cambridge, UK Shows that multiplexed biomedical fluorescence imaging could enable highly sensitive and specific diagnosis, providing the information required for personalized image-guided treatment Discusses spectrally resolved detector arrays (SRDAs), which provide a compact and robust approach to multiplexed biomedical fluorescence imaging Details camera characterizations, calibrations, and system integrations required when implementing spectrally resolved detector arrays in biomedical imaging instrumentation
Autor*in
Anna Siri Luthman
Themen in »Spectrally Resolved Detector Arrays for Multiplexed Biomedical Fluorescence Imaging«
Hyperspectral Imaging Multispectral Imaging Spectrally Resolving Detector Arrays Camera Calibration Endoscopy In Vivo Imaging