Biotechnologies for Plant Mutation Breeding
Protocols
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Beschreibung
This book is open access under a CC BY-NC 2.5 license.
This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
This book is open access under a CC BY-NC 2.5 license.
This book offers 19 detailed protocols on the use of induced mutations in crop breeding and functional genomics studies, which cover topics including chemical and physical mutagenesis, phenotypic screening methods, traditional TILLING and TILLING by sequencing, doubled haploidy, targeted genome editing, and low-cost methods for the molecular characterization of mutant plants that are suitable for laboratories in developing countries. The collection of protocols equips users with the techniques they need in order to start a program on mutation breeding or functional genomics using both forward and reverse-genetic approaches. Methods are provided for seed and vegetatively propagated crops (e.g. banana, barley, cassava, jatropha, rice) and can be adapted for use in other species.
Open Access provided with a grant from the International Atomic Energy Agency All contributions have been peer-reviewed An excellent reference source for plant breeders, geneticists and seed companies as well as for undergraduate and postgraduate students specializing in agriculture, biotechnology, and molecular breeding
Autor*in
Joanna Jankowicz-Cieslak
Themen in »Biotechnologies for Plant Mutation Breeding«
TILLING Embryonic plant cells Mutation detection Plant mutagenesis Random mutagenesis Site-directed mutagenesis Mutation induction Chimera dissociation Phenotypic screening Genotypic screening genetic engineering