This thesis offers a unique guide to the development and application of ultrasensitive optical microscopy based on light scattering. Divided into eight chapters, it covers an impressive range of scientific fields, from basic optical physics to molecular biology and synthetic organic chemistry. Especially the detailed information provided on how to design, build and implement an interferometric scattering microscope, as well as the descriptions of all instrumentation, hardware interfacing and image processing necessary to achieve the highest levels of performance, will be of interest to researchers now entering the field.
Nominated as an outstanding PhD thesis by the University of Oxford, UK
Bridges the gaps between multiple disciplines
Provides unique insights into fundamental questions concerning molecular motors, membrane biophysics, single protein detection, condensed matter physics, and synthetic chemistry
Presents detailed information on how to design, build and implement an interferometric scattering microscope, including hardware interfacing and the various stages of data processing
Jaime Ortega Arroyo
High Speed Single-particle Tracking Interferometric Scattering Microscopy (iSCAT) Label-free Single-protein Detection Label-free Imaging Myosin 5a Dynamics Membrane Biophysics Ultrasensitive Optical Microscopy Physical Autocatalysis Single-molecule Approaches to Optical Microscopy