Multi-Parametric Live Cell Microscopy of 3D Tissue Models
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Beschreibung
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.
Provides easy-to-follow protocols for specialists in cancer biology and tissue engineering Details current achievements in design and application of probes, dye, and nanoparticles as well as sensors for live cell imaging of 3D tissue models Consolidates cross-disciplinary knowledge of modern microscopy with tissue engineering approaches Includes supplementary material: sn.pub/extras
Autor*in
Ruslan I. Dmitriev
Themen in »Multi-Parametric Live Cell Microscopy of 3D Tissue Models«
3D live cell imaging 3D tissue models Applications Multi-parametric imaging Autofluorescence imaging in cancer Ca2+ imaging neural Multi-parametric imaging cancer Multi-parametric imaging of pH Multi-parametric imaging viscosity cancer Multi-parametric quantitative imaging Quantitative imaging intestinal organoid Biological Microscopy