Single-Molecule Metal-Induced Energy Transfer
From Basics to Applications
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Beschreibung
This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.
This thesis presents a novel single-molecule spectroscopy method that, for the first time, allows the dipole orientations and fluorescence lifetimes of individual molecules to be measured simultaneously. These two parameters are needed to determine the position of individual molecules with nanometer accuracy near a metallic structure. Proof-of-principle experiments demonstrating the value of this new single-molecule localization concept are also presented. Lastly, the book highlights potential applications of the method in biophysics, molecular physics, soft matter and structural biology.
Nominated as an outstanding PhD thesis by Göttingen University, Germany Introduces the electrodynamics of a dipole emitter from fundamentals with illustrations Includes descriptions of the optical setups used, together with possible modifications/combinations that will be especially useful for experimental physicists Features discussion/comments on the practical aspects of the method, highlighting its potential applications in biophysics, structural biology, and molecular physics Includes supplementary material: sn.pub/extras
Autor*in
Narain Karedla
Themen in »Single-Molecule Metal-Induced Energy Transfer«
Superresolution Microscopy Axial Resolution Förster Resonance Energy Transfer Transition Dipole Pattern Matching Fluorescence Lifetime Imaging Microscopy Single-molecule Imaging Defocused Imaging Single-molecule Localization Microscopy