Rapid Cell Magnetisation Using Cationised Magnetoferritin
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Beschreibung
Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
Bringing together nanoscience with stem cell and bacterial cell biology, this thesis is truly interdisciplinary in scope. It shows that the creation of superparamagnetic nanoparticles inside a protein coat, followed by chemical functionalisation of the protein surface, provides a novel methodology for cell magnetisation using incubation times as short as one minute. Crucially, stem cell proliferation and multi-lineage differentiation capacity is not impaired after labelling. Due to the unspecific labelling mechanism, this thesis also shows that the same magnetic protein nanoparticles can be used for rapid bacterial magnetisation. Thus, it is possible to magnetically capture and concentrate pathogens from clinical samples quickly and highly efficiently.
Nominated as an outstanding PhD thesis by the University of Bristol, Bristol, UK Presents a comprehensive introduction to magnetic nanoparticles and their use in magnetic cell labelling Includes a detailed protocol describing the synthesis and functionalisation of novel, protein-based magnetic nanoparticles for ultrafast and universal cell labelling Discusses in-depth the characterisation of magnetic labelling efficiency and the effects on stem cell biology Includes supplementary material: sn.pub/extras
Autor*in
Sara Correia Carreira
Themen in »Rapid Cell Magnetisation Using Cationised Magnetoferritin«
Magnetic Nanoparticles Magnetoferritin Protein Modification Stem Cell Magnetization Magnetic capture of pathogens Magnetic Cell Separation Bacteria Isolation Bacteria Magnetisation Magnetic labelling of biological cells