Decoding the Antibody Repertoire
High Throughput Sequencing of Multiple Transcripts from Single B Cells
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Beschreibung
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening the door for the discovery of new antibodies and the investigation of adaptive immune responses to vaccines and diseases. By designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells, the author directly addresses the limitations to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. Previous methods for high-throughput immune repertoire sequencing have been unable to provide such information. The techniques developed in this thesis have enabled comprehensive investigation of human B-cell repertoires and have been applied for the rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
This thesis outlines the development of the very first technology for high-throughput analysis of paired heavy and light-chain antibody sequences, opening an entirely new window for antibody discovery and the investigation of adaptive immune responses to vaccines and diseases.
Previous methods for high-throughput immune repertoire sequencing have been unable to provide information on the identity of immune receptor pairs encoded by individual B or T lymphocytes. The author directly addresses these limitations by designing two new technologies for sequencing multiple mRNA transcripts from up to 10 million isolated, single cells.
The techniques developed in this work have enabled comprehensive interrogation of human B-cell repertoires and have been applied for rapid discovery of new human antibodies, to gain new insights into the development of human antibody repertoires, and for analysis of human immune responses to vaccination and disease.
Nominated as an outstanding Ph.D thesis by the University of Texas at Austin Outlines the development of the very first technology for high-throughput analysis of paired heavy and light chain antibody sequences from Enables comprehensive investigation of human B-cell repertoires for rapid antibody discovery Includes supplementary material: sn.pub/extras Includes supplementary material: sn.pub/extras
Autor*in
Brandon DeKosky
Themen in »Decoding the Antibody Repertoire«
B Cell Single-cell Sequencing Antibody Immunology Immune Repertoire High-throughput Sequencing Next Generation Sequencing B Cell Receptor Antibody Discovery antibodies genetic engineering