Low-Cost Methods for Molecular Characterization of Mutant Plants
Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Buch in deiner Nähe kaufen
Beschreibung
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
This book offers low-cost and rapid molecular assays for the characterization of mutant plant germplasm. Detailed protocols are provided for the desiccation of plant tissues; the extraction of high-quality DNA for downstream applications; the extraction of single-strand-specific nucleases for single nucleotide polymorphism; and small insertion/deletion discovery using standard agarose gel electrophoresis. The methods described can be applied in any laboratory equipped for basic molecular biology and do away with the need for expensive freezers and toxic organic compounds. With the appropriate validation of sample quality and longevity, they can provide sufficient DNA for a variety of molecular applications, such as marker studies and TILLING, at approximately one tenth of the cost per sample when compared to commercial kits.
Open Access provided with a grant from the International Atomic Energy Agency Describes low-cost and easy-to-use assays for the molecular characterization of mutant plant material The experimental protocols do away with the need for complicated procedures, expensive equipment and hazardous chemicals All protocols included have been tried and tested by researchers all over the world Includes supplementary material: sn.pub/extras
Autor*in
Bradley J. Till
Themen in »Low-Cost Methods for Molecular Characterization of Mutant Plants«
Crop improvement DNA extraction Induced mutations Low-cost assays Mutant germplasm characterization Nucleotide variation in plant genomes PCR amplification