Mitochondrial DNA
Methods and Protocols
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Beschreibung
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mitrochondrial DNA: Methods and Protocols, Second Edition promises to aid researchers in further expanding our knowledge of this vital area of cell biology.
Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mitrochondrial DNA: Methods and Protocols, Second Edition promises to aid researchers in further expanding our knowledge of this vital area of cell biology.
Covers a wide range of methods for investigating how mitochondrial genetics affect cellular physiology and human health Details unique experimental approaches for studying mtDNA damage and mutation An experimental resource for researchers and physicians concerned with mtDNA disorders Provides an in depth description of tools and approaches used by leading researchers A comprehensive compendium of tools and approaches used in the field
Autor*in
Jeffrey A Stuart
Themen in »Mitochondrial DNA«
DNA Damage and repair Disorders Heteroplasmic PCR Reactive oxygen species (ROS) Replication and transcription cell biology genetics human cell lines molecular biology mtDNA mutations saccharomyces cerevisiae tissue transcription
Stimmen zu »Mitochondrial DNA«
From the reviews of the second edition:
“The book contains detailed descriptions both of established techniques that continue to be usefully applied and recent information that improves understanding of mtDNA biology. It is intended for graduate students, postdoctoral fellows, and established investigators. I think even teachers in this field and early career investigators will find this book quite useful.” (Omer Iqbal, Doody’s Review Service, January, 2010)
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