Nucleic Acid and Peptide Aptamers
Methods and Protocols
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Beschreibung
After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In Nucleic Acid and Peptide Aptamers: Methods and Protocols, expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, Nucleic Acid and Peptide Aptamers: Methods and Protocols will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular Biology™ series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.
Provides an easily accessible reference volume for in vitro selection procedures written by leading researchers in the field Covers a wide range of in vitro selection protocols targeting different molecules Comprehensive guide to identifying your specific target binding aptamer, whether of nucleic acid or peptidic nature Detailed presentation of applications of aptamers The first comprehensive summary that covers peptide and nucleic acid aptamers in one volume Includes supplementary material: sn.pub/extras
Autor*in
Günter Mayer
Themen in »Nucleic Acid and Peptide Aptamers«
Ligands Protein modification RNA Target molecules genes in vitro methods molecular biology
Stimmen zu »Nucleic Acid and Peptide Aptamers«
From the reviews:
"The book ‘Nucleic Acids and Peptide Aptamers’ is a collection of detailed protocols describing various aspects related to generating aptamers, characterizing them, and applying for particular purposes. … The list of references is a useful and timely source of further reading on the subject. … can be certainly recommended as an excellent handbook addressed to researchers and students in the molecular biology field." (Ryszard W. Adamiak and Mikolaj Olejniczak, Acta Biochimica Polonica, Vol. 56 (3), 2009)
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