Fluorescence Spectroscopy and Microscopy
Methods and Protocols
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Beschreibung
Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.
Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.
Explores the methods and applications in the expanding fluorescence field Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts
Autor*in
Yves Engelborghs
Themen in »Fluorescence Spectroscopy and Microscopy«
Cuvette-based studies Fluorescence Fluctuation Spectroscopy Fluorescence Lifetime Imaging Fluorescence Recovery After Photobleaching Fluorescent Probe Development Förster Resonance Energy Transfer Imaging Green fluorescent protein (GFP) Single-Molecule Fluorescence Spectroscopy Steady-State Fluorescence Spectroscopy Time-Resolved Fluorescence Spectroscopy Biological Microscopy
Stimmen zu »Fluorescence Spectroscopy and Microscopy«
From the reviews:
“The book is illustrated with high-quality figures in both color and black and white. … This book offers techniques from internationally recognized experts. Both novice and established scientists will find it extremely useful.” (Omer Iqbal, Doody’s Book Reviews, March, 2014)()