siRNA Design
Methods and Protocols
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Beschreibung
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results.
Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.
The discovery of RNA interference (RNAi) as a methodology for gene silencing has revolutionized biological research, providing an invaluable avenue for therapeutics, and small interfering RNA (siRNA) is the most common strategy utilized for enacting RNAi. siRNA Design: Methods and Protocols offers expertly crafted guidelines and protocols for the selection of siRNA targeting sequences, for the strategic incorporation of chemical modifications, and for advantageous structural modifications to the classic siRNA design. Protocols are provided for using endogenous cellular machinery to produce siRNA from optimized precursor short hairpin RNA (shRNA) and artificial microRNA (amiRNA) molecules. Strategies are also described for specific applications such as immunostimulatory siRNA that may provide therapeutic benefit against viral infections in mammals, the simultaneous targeting of multiple siRNAs, and siRNA-mediated crop virus resistance. The design of RNAi for gene silencing in embryonic, invertebrate, and plant systems requires a variety of unique approaches, several of which are described towards the end of this volume. Written for the highly successful Methods in Molecular Biology™ series, this work contains the kind of detailed description and implementation advice that guarantees successful results.
Authoritative and easy to use, siRNA Design: Methods and Protocols will provide researchers, educators, clinicians, and biotech specialists with a broad understanding of the issues in RNAi and how they can be overcome strategically through design.
Assists researchers, educators, clinicians, and biotech scientists in harnessing the power of RNAi Features easy to follow protocols and techniques for siRNA design Includes expert tips and implementation advice to ensure successful results Includes supplementary material: sn.pub/extras
Autor*in
Debra J. Taxman
Themen in »siRNA Design«
RNAi Short hairpin RNA (shRNA) Small interfering RNA Therapeutic applications siRNA design strategy siRNA effector molecules siRNA target sequences
Stimmen zu »siRNA Design«
From the reviews:
“This book edited by D.J. Taxman is a valuable collection of 20 chapters covering aspects from values for parameters used in tools for designing siRNAs to screening methods for evaluating the efficiency of the designed siRNA. … Through style and scientific information the book is aimed at researchers in the RNAi field, but its accessible delivery of contents makes it a good starting point for younger researchers in this topic.” (Irina Ioana Mohorianu, zbMATH, Vol. 1276, 2014)()