DNA-Protein Interactions
Principles and Protocols, Third Edition
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Beschreibung
Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions. Written in the convenient and informative Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, DNA-Protein Interactions: Principles and Protocols, Third Edition serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research.
Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions. Written in the convenient and informative Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, DNA-Protein Interactions: Principles and Protocols, Third Edition serves as an ideal guide for all those exploring this dynamic, essential, and increasingly affordable area of research.
Covers cutting edge techniques in chromatin studies, such as Nucleosome Mapping, Chromatin Immunoprecipitation and ChIP-on-chip Contains many protocols for large scale analysis of genome-protein interaction and long-distance interaction between chromatin domains Describes the use of many physical techniques like Atomic Force Microscopy, FRET, Surface Plasmon Resonance and Circular Dichroism Accessible and highly usable protocols for in vivo and in vitro Footprinting, Electromobility Shift Assays, and other proven DNA-protein interaction assays Offers authors' comments on delicate manipulations, information that rarely makes it into ordinary protocols
Autor*in
Tom Moss
Themen in »DNA-Protein Interactions«
Chromatin immunoprecipitation DNA Genome-wide techniques In vivo techniques Microarray PCR Photocrosslinking Termination Western Blotting gene expression genes microscopy molecular biology protein complexes transcription
Stimmen zu »DNA-Protein Interactions«
Reviews of Previous Editions:
"The book, written by an impressive list of worldwide contributors, will provide readers with nearly all the necessary tools for studying DNA-protein interactions, making it a perfect cell laboratory manual." -Quarterly Review of Biology
"...a great laboratory resource for biochemists and molecular, cellular, and developmental biologists." - Today's Life Science
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