Alcohol
Methods and Protocols
Buch in deiner Nähe kaufen
Beschreibung
Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure, and its contribution to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics. As part of the successful Methods in Molecular Biology™ series, each chapter provides step-by-step laboratory protocols, lists of the necessary materials, and a Notes section with tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly specific, Alcohol: Methods and Protocols strives to further spur investigators to delve into the complex and fascinating processes of the adaptive and maladaptive responses humans have developed to the consumption of alcohol.
Many unexplored complex cellular and organismal adaptations occur in response to the stress of alcohol exposure. The contribution of alcohol exposure to the development of chronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In this book, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Comprehensive and highly specific, this book strives to further spur investigators to delve into the complex and fascinating processes of the adaptive and maladaptive responses humans have developed to the consumption of alcohol.
Step-by-step protocols for the most commonly used models of acute and chronic ethanol exposure Detailed procedures for cell culture experiments with ethanol Comprehensive methods for studies of fetal alcohol syndrome (FAS) including Zebrafish, avian embryo and rodent models Up-to-date proteomics and micro-array protocols Specific protocols of interest to neuroscientists, immunologists, physiologists, cell biologists and biochemists Includes supplementary material: sn.pub/extras
Autor*in
Laura E. Nagy
Themen in »Alcohol«
Adaptive and maladaptive response Antigen Cell and tissue development Chronic ethanol exposure Ethanol Excessive alcohol consumption Microarray Pathophysiology Regulation bone cells gene expression genes tissue