Adenovirus Methods and Protocols
Volume 1: Adenoviruses, Ad Vectors, Quantitation, and Animal Models
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Beschreibung
Adenovirus Methods and Protocols, Second Edition, now in two volumes, is an essential resource for adenovirus (Ad) researchers beginning in the field, and an inspirational starting point for researchers looking to branch into new areas of Ad study. In addition to updating and expanding important chapters from the first edition, the authors have added new chapters that address innovative, exciting areas of emphasis in Ad research. Each of the protocols presented in these volumes is written by trendsetting researchers in their respective areas of expertise. The protocols provide a general introduction, followed by tried-and-true step-by-step methods. Both novice and experienced researchers will reap tremendous benefit from these groundbreaking volumes in Ad research.
Detailed description of quality control testing of vectors Methods for determination of virus titers Sensitive assays for testing viral DNA presence in samples Developing new, permissive immunocompetent animal models Characterization of specific Ad proteins (hexon, fiber, or protease/proteinase) Procedures for microinjection, transformation assays, and NK cell-mediated cytolysis Analysis of phylogenetics and genetic variability Includes supplementary material: sn.pub/extras
Autor*in
William S. M. Wold
Themen in »Adenovirus Methods and Protocols«
DNA ELISA Escherichia coli PCR Viruses apoptosis genes
Stimmen zu »Adenovirus Methods and Protocols«
"...provdes some good advice on using and growing adenovirus vectors." -Doody's Book Review, Weighted Numerical Score: 62 - 2 Stars
Reviews of the First edition:
"This book caters quite well to those who simply want to grow adenoviruses, assay them, and use them. . .a very comprehensive methods book that will be a laboratory standard for years to come."-Microbiology Today
". . .this book constitutes an excellent reference text . . .this book is replete with useful information for both the virologist and the non-virologist who is interested in working with adenoviruses."-FEBS Letters
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