Cryopreservation and Freeze-Drying Protocols
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Beschreibung
This widely expanded second edition offers a compilation of robust, reproducible techniques for the conservation of a wide range of biological materials. It includes novel approaches and protocols that were not preservable when the first edition was published.
The book begins with a discussion of long term ex situ conservation of biological resources, the role of biological resource centers, and fundamental principles of freeze-drying and cryopreservation. Each chapter focuses on the preservation of specific biological materials, including proteins, mircroorganisms, cell lines, and multicellular structures. Coverage presents detailed descriptions of the materials required and stepwise protocols for each preservation method, along with explanatory notes that highlight key technical issues. The troubleshooting and background notes provided for each protocol enhance this practical and reliable resource for the conservation and sustainable use of biological resources.
Background and explanatory notes for each protocol Methods for lyophilization of proteins Cutting-edge methods for conservation and sustainable use of resources New and novel techniques for preserving biological materials
Autor*in
John G. Day
Themen in »Cryopreservation and Freeze-Drying Protocols«
biological materials biological resource centers biological resource centres fish sperm human cell lines human embryonic stem cell lines lyophilization mammalian semen multicellular structures prokaryotes red blood cells reproducible techniques shelf-freeze-drier vacuum-drying yeast cultures
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From the reviews of the second edition:
"...practical and reliable resource for the conservation and sustainable use of biological resources."-Anticancer Research
"This book is a welcome addition to the relatively sparse comprehensive reference publications on preservation methodologies which are currently available. It begins sensibly with a grounding in the principles involved in preservation, in logical and easy to follow steps. It is well laid out and covers a wide spectrum of cell/organism types in the one volume … ." (Peter Green, Molecular Biotechnology, December, 2007)
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