Cardiac Gene Expression
Methods and Protocols
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Beschreibung
Cardiac Gene Expression: Methods and Protocols presents both cutting-edge and established methods for studying cardiac gene expression. The protocols provide a template for solid research, and cover the process through screening, analysis, characterization, and functional confirmation of novel genes or known genes with a new function.
Section I, Cardiac Gene Expression Profiling: The Global Perspective, discusses several different approaches to examining, identifying, and analyzing changes in transcriptome gene expression. Section II, Cardiac Gene Regulation: Gene-Specific mRNA Measurement in the Myocardium, outlines more sensitive and gene-targeted expression methods. Section III, Cardiac Gene Regulation: Promoter Characterization in the Myocardium, provides protocols for the study of underlying gene regulation mechanisms by focusing on the interaction of transcription factors with their cognate cis binding elements. Section IV, In Silico Assessment of Regulatory cis-Elements and Gene Regulation, and Section V, Cardiac Single Network Polymorphisms, emphasize new analytical approaches for deciphering the functional elements buried in the 3 billion nucleotides of the human genome and other model genomes. The concluding section, Gene Overexpression and Targeting in the Myocardium, highlights methods that facilitate overexpression or cardiac-specific targeted gene deletion.
Resources for the analysis of gene regulation data and SNPs Comparative genomic approach for functionally annotating human DNA Quantitative, real-time RT-PCR in cardiovascular research In silico analysis of SNPs and other high-throughput data Conditional targeting in the myocardium Includes supplementary material: sn.pub/extras
Autor*in
Jun Zhang
Themen in »Cardiac Gene Expression«
DNA In silico Microarray PCR Polymerase Chain Reaction Promoter SNP Single Nucleotide Polymorphism gene expression gene transfer genes genome hybridization regulation mechanisms transcription
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"...a timely, detailed and well edited methodss text which would be a valuable addition to any cardiovascular research laboratory." -Molecular Biotechnology
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