In Vitro Mutagenesis Protocols
Third Edition
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Beschreibung
In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
In the post-genomic era, in vitro mutagenesis has emerged as a critically important tool for establishing the functions of components of the proteome. The third edition of In Vitro Mutagenesis Protocols represents a practical toolbox containing protocols vital to advancing our understanding of the connection between nucleotide sequence and sequence function. Fully updated from the previous editions, this volume contains a variety of specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein structure-function, protein regulation of biological processes, and protein activity, as well as a novel section on mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, In Vitro Mutagenesis Protocols, Third Edition offers today's researchers a valuable compendium of reliable and powerful techniques with which to illuminate the proteome and its rich web of biological implications.
Establishes protein structure, function, activity, and interrelationships by authors of peer reviewed articles published in respected journals Covers computational methods to evaluate protein stability and the resultant change upon introducing mutations Utilizes animal models to identify loci that genetically interact with human disease-causing point mutations Includes detailed methods on protein structure and function mutagenesis Includes supplementary material: sn.pub/extras
Autor*in
Jeff Braman
Themen in »In Vitro Mutagenesis Protocols«
BAC Chromosom DNA Escherichia coli Microbial systems Nucleotide sequences PCR Protein regulation Protein structure-function Protein-protein interaction Regulation Sequence function molecular biology protein structure transcription
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From the reviews of the third edition:
“This well-illustrated book details specialty tools successfully employed to unravel the intricacies of protein-protein interaction, protein-structure function, protein regulation of biological processes, and protein activity and mutagenesis methods for unique microbes as a guide to the generalization of mutagenesis strategies for a host of microbial systems. … Scientists, students, and others interested in the field of in vitro mutagenesis will find this extremely useful. … This book successfully describes a variety of in vitro mutagenesis protocols for scientists working in this area.” (Omer Iqbal, Doody’s Review Service, November, 2010)()