RT-PCR Protocols
Second Edition
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Beschreibung
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.
User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.
Features all the latest and hottest technology used in conventional and real-time RT-PCR Provides a broad scope covered in good depth, from detection of minute qualities of microRNA to the analysis of complex cell populations Serves as a comprehensive guide to setting up, running and analyzing an RT-PCR experiment
Autor*in
Nicola King
Themen in »RT-PCR Protocols«
Cloning Gene sequences PCR Primer design Real-time quantitative RT-PCR Reverse transcription Reverse-transcription polymerase chain reaction developmental biology gene expression genes molecular biology transcription
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From the reviews of the second edition:
“The second edition of RT-PCR protocols and attempts to cover the most commonly used RT-PCR protocols, giving both beginners and experts a deeper understanding of the experimental set-up. … There are often flow diagrams or pictures to aid understanding and the references are helpful, if you require additional information. … a good investment for any lab interested in ensuring that their RT-PCR reactions are as productive and well-controlled as possible. … Overall a useful and detailed text written in an informative and helpful style.” (Hayley Evans, Immunology News, May, 2011)()