Molecular Epidemiology of Microorganisms
Methods and Protocols
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Beschreibung
The development of molecular genotyping methods has revolutionized the possibility for classification of microorganisms at the sub-species level. This investigation of species diversity is crucial for deciding the molecular relatedness of isolates for epidemiological studies. In Molecular Epidemiology of Microorganisms: Methods and Protocols, readers will find readily reproducible methods for determining the molecular epidemiology of microorganisms, all of which provide effective discrimination of unrelated strains. This volume covers a wide range of techniques which can be easily applied to the investigation of transmissible diseases, directing researchers towards the most effective methods based on the particular information to be obtained. Describing both traditional and novel techniques, expert researchers present a series of methods-based chapters with applications to some of the most important microbes. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Molecular Epidemiology of Microorganisms: Methods and Protocols provides state-of-the-art techniques which will prove to be critical in unraveling the routes of spread of pathogens for humans, animals, and plants.
The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level. Investigation of the species diversity is required to determine molecular relatedness of isolates for epidemiological studies. Methods for molecular epidemiology of microorganisms must be highly reproducible and provide effective discrimination of epidemiologically unrelated strains. A wide range of techniques has been applied to the investigation of outbreaks of tra- missible disease, and these have been critical in unraveling the route of spread of pat- gens for humans, animals, and plants. The choice of a molecular method will depend on the type of questions to be addressed, on the degree of genetic diversity of the species to be analyzed, and on the mechanisms responsible for generation of the diversity. The applications of molecular methods, singly or in combination, have greatly contributed in the past two decades to basic microbial science and public health control strategies. Molecular Epidemiology of Microorganisms: Methods and Protocols brings together a series of methods-based chapters with examples of application to some of the most important microbes. Both traditional and novel techniques are described, and the type of information that can be expected to be obtained by their application is indicated.
Includes cutting-edge methods for the epidemiology of microorganisms Provides a comparative overview of methods best adapted to species diversity Includes tools for advanced data analyses and interpretation
Autor*in
Dominique A. Caugant
Themen in »Molecular Epidemiology of Microorganisms«
Bacterial genome sequencing Epidemiological Multilocus sequence typing Multiple locus VNTR Pulsed-field gel electrophoresis Randomly amplified DNA analysis Rep-PCR Spa-typing Viral gene sequencing molecular epidemiology
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From the reviews:
"This book provides valuable insight into the molecular genotyping methods for differentiation and classification of microorganisms at the subspecies level … . It is intended for researchers in the field of molecular epidemiology of microorganisms. Students as well as experts in the field will find this book quite informative and useful. … This is a valuable resource providing state-of-the-art techniques applied to the investigation of transmissible diseases, enabling researchers to unravel the routes of spread of pathogens for humans, animals, and plants." (Omer Iqbal, Doody’s Review Service, September, 2009)
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