Chromatin Immunoprecipitation Assays
Methods and Protocols
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Beschreibung
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology.
Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell’s biology.
Provides an up-to-date reference volume for chromatin immunoprecipitation assays written by leading researchers in the field Delivers easily accessible and thoroughly tested bench protocols Covers a wide range of biological systems, species and state-of-the-art approaches to analyses A comprehensive guide to developing, running and analyzing chromatin immunoprecipitation-based assays for the study of protein-DNA interactions Includes supplementary material: sn.pub/extras
Autor*in
Philippe Collas
Themen in »Chromatin Immunoprecipitation Assays«
ChIP DNA DNA microarray Genome-wide datasets High-throughput sequencing Protein binding SNP Termination chromosome genetics molecular biology transcription microarrays
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From the reviews:
“This is a very useful book for researchers who utilise chromatin Immunoprecipitation (ChIP). … In conclusion, the chapters in this book describe the methods in detail and in general they have reasonable bibliographies. This book is a very useful acquisition for researchers who are embarking upon research that employs ChIP and equally for those researchers who are already undertaking it … .” (Ray Waters, Human Genetics, Vol. 127, March, 2010)()