Retinoids
Methods and Protocols
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Beschreibung
New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A’s impact on the human body.
New and exciting biological functions are still being discovered for vitamin A derivatives, including the vast number of physiological activities of retinoids. In Retinoids: Methods and Protocols, expert researchers in the field present the most recent technical tools with diverse techniques for both in vitro and in vivo studies. Combining biochemical, biophysical, and cell biological techniques, the book addresses topics such as the detection and quantitation of retinoids using HPLC, mass spectrometry, and fluorescence, fluorescence anisotropy of retinol binding protein, cell culture models for studying retinoid transport and the role of retinol in embryonic stem cell culture, as well as many other detailed procedures. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Retinoids: Methods and Protocols seeks to aid beginning and experienced researchers from widely varied fields in the search to uncover even more vital aspects of vitamin A’s impact on the human body.
Comprehensive technical guides to roles of vitamin A and retinoids in diverse physiological and pathological states and molecular pathways Up-to-date descriptions of biochemical, biophysical, and cell biological techniques Easily accessible reference volume for beginners and experts Includes supplementary material: sn.pub/extras
Autor*in
Hui Sun
Themen in »Retinoids«
Transport Vitamin Vivo biological functions cells enzymes hybridization molecular biology tissue
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From the reviews:
“This excellent book provides authoritative and cutting-edge information in the field of retinoids … . It is intended both for beginning and experienced researchers in diverse fields who are interested in learning new techniques. Even graduate students, nutritionists, and clinicians could find this book informative and helpful. … By combining biochemical, biophysical, and cell biological techniques, the book thoroughly addresses the field of retinoid research.” (Omer Iqbal, Doody’s Review Service, October, 2010)()