Protein Blotting and Detection
Methods and Protocols
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Beschreibung
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In "Protein Blotting and Detection: Methods and Protocols", expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
Includes 52 different protein transfer and detection techniques Gives a brief overview of 35 additional protein transfer and detection methodologies Provides a history of protein transfer development and an overview of membrane proteomics Outlines inexpensive protein blotting and detection protocols useful for large college courses Allows Investigators to rapidly implement a technique that is new to them and to their laboratories Includes supplementary material: sn.pub/extras
Autor*in
Biji T. Kurien
Themen in »Protein Blotting and Detection«
Immunoblotting Membrane proteomics Polyacrylamid-Gelelektrophorese Protein transfer Proteomics SDS-PAGE TLC-Blot Western blotting molecular biology
Stimmen zu »Protein Blotting and Detection«
From the reviews:
"This informative, hands-on-guide is essential for all investigators interested in learning techniques based on Western blotting which they can easily transfer to their laboratories. … The book is intended for any investigator interested in learning a new technique based on Western blotting. … this hands-on guide will be quite useful and helpful for both investigators and students working in these areas." (Omer Iqbal, Doody’s Review Service, October, 2009)
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