Circadian Rhythms
Methods and Protocols
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Beschreibung
The aim of Circadian Rhythms is to provide a resource that can be adopted by several types of users: those who are new to circadian biology, those who are already active in the field but are interested in learning new techniques and researchers who are considering moving to a new a model system or undertaking comparative studies and would like to consult protocols applied to different organisms before starting the study of new species. This book features a full range of methods that illustrate procedures that have been recently been introduced in circadian studies and by presenting variations to take into account the peculiarities of different model systems.
Methods for analysis of rhythmic readouts in select model organisms Novel techniques for identifying clock genes by mutagenesis and mircroarrays Procedures for isolation, extraction, and analysis of RNA Procedures for extraction, fractionation, and purification of proteins Detailed in vitro protocols for circadian research Methods for microscopy analysis in circadian biology Includes supplementary material: sn.pub/extras
Autor*in
Ezio Rosato
Themen in »Circadian Rhythms«
Arabidopsis thaliana Microarray PCR Quantitative PCR Vivo Western Blotting cell lines cyanobacteria gene expression genes hybridization mammalian cell lines