High Throughput Protein Expression and Purification
Methods and Protocols
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Beschreibung
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
Despite exciting advances in genome sequencing, isolating a protein from its expression system in its native form still presents a complex challenge. In High Throughput Protein Expression and Purification: Methods and Protocols, leading scientists detail the most successful protocols currently in use, including various high throughput cloning schemes, protein expression analysis, and production protocols. This volume describes the use of E. coli, insect, and mammalian cells, as well as cell-free systems for the production of a wide variety of proteins, including glycoproteins and membrane proteins, in order to best represent strategies that create and exploit common features to enable simplified cloning, stable expression, and purification of proteins. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and a Notes section for tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, High Throughput Protein Expression and Purification: Methods and Protocols is an ideal reference for protein biochemists and all those who wish to apply these easy-to-use protocols to the many applicable fields.
Provides step-by-step protocols for protein production that utilize a wide range of cutting-edge techniques Covers cell-based and cell-free methodologies needed to produce bacterial proteins, eukaryotic proteins, soluble and membrane proteins Examines a variety of cloning methodologies that enable high-throughput cloning for protein production Describes the use of a variety of tags to facilitate parallel processing for high-throughput protein production Includes several reviews to help readers choose which protocols best suits their needs Provides protocols that utilize robotics for sample handling, as well as protocols designed small laboratories performing experiments by hand
Autor*in
Sharon A. Doyle
Themen in »High Throughput Protein Expression and Purification«
Cloning Escherichia coli Genome sequencing PCR Parallel processing membrane proteins molecular biology proteins Protein Structure
Stimmen zu »High Throughput Protein Expression and Purification«
From the reviews:
"The book consists 20 chapters, which cover methods in protein expression and purification summarized by leading experts in this area from USA, UK, and Switzerland. … Numerous pictures in the text, a references list at the end of each chapter, and a subject index help better understand the presented materials. The volume is true handbook for protein biochemists, molecular biologists, biotechnologists, and other researchers and technicians who are involved in the many applicable fields." (G. Wiederschain, Biochemistry (Moscow), Vol. 74 (8), 2009)
“High Throughput Protein Expression and Purification: Methods and Protocols, edited by Sharon A. Doyle, is an interesting attempt showing what one currently has at one’s disposal if one wishes to make a first step toward this aim. … this book should be recommended especially to researchers who would like to organize a new laboratory focused on high throughput protein production, or to improve an existing one. It can also prove helpful to those who wish to optimize individual protein expression, isolation or purification.” (Marek Figlerowicz, Acta Biochimica Polonica, Vol. 56 (4), December, 2009)()