Glycomics
Methods and Protocols
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Beschreibung
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
Presents a detailed compendium of techniques used routinely in the leading laboratories utilizing current, common technologies, especially those established for proteomics research Reviews the current practices used for the analysis of the specific single O-GlcNAc found on nuclear and cytoplasmic proteins Disperses the misconception that only experts can analyze the glycome Inspires researchers to pursue glycan function through sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools Includes supplementary material: sn.pub/extras
Autor*in
Nicolle H. Packer
Themen in »Glycomics«
Microarray Oligosaccharid Polyacrylamid-Gelelektrophorese Termination molecular biology proteins proteoglycans carbohydrate chemistry
Stimmen zu »Glycomics«
From the reviews:
“This book discusses methods of a comparatively new direction in science, which has been named glycomics. … The book consists two parts with 26 chapters prepared by well-known experts from many countries. … I confirm the book fulfills the goal and I consider this book as an excellent practical guide to glycomics methods. Each method is described in great detail, and the whole book is excellent manual for many researchers in biochemistry, molecular biology, immunochemistry, biotechnology, and medicine.” (G. Wiederschain, Biochemistry, Vol. 75 (4), 2010)
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