In Situ Hybridization Protocols

Beschreibung

In In Situ Hybridization, Third Edition, Ian Darby updates his highly successful second edition and shifts the focus to tissue and cell in situ hybridization. Drawing on experts working in diverse areas and taking advantage of advances in non-isotopic in situ hybridization and amplification techniques, the authors describe in detail their readily reproducible techniques for preparation, detection, and tissue hybridization. Among the new techniques detailed are PNA probes for viral diagnostics, plant in situ hybridization, cell proliferation detection, and quantitation of in situ hybridization. There are also cutting-edge techniques for tissue microarrays, expanded embryology-developmental gene detection, and expanded cell culture. Derivative techniques presented include the identification of transplanted cells, histones, nick-end labeling for apoptosis, the use of peptide nucleic acid probes, and in situ hybridization of plant specimens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Completely updated and highly practical, In Situ Hybridization, Third Edition allows both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use these powerful new techniques successfully.
The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
Includes supplementary material: sn.pub/extras
In Situ Hybridization has successfully provided basic and advanced laboratory techniques. With increasing technology and evolving applications, this edition concentrates on in situ hybridization of cells and tissues and the derivative techniques that have not been covered in the previous editions. The third edition will be a valuable aid in setting up the technique or improving the sensitivity and scope of applications of in situ hybridization in their own laboratories.

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Ian A. Darby

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Reviews of Previous Editions:

"...an indispensable laboratory manual for the current techniques used...essential for the current techniques used...essential for any laboratory that is using, or wants to use, the...technique in their repertoire."-Molecular Biotechnology

" . . .a good book for anyone wishing to exploit all that in situ hybridization has to offer."
-- Biopharm Manufacturing
Reviews for Second Edition...
"This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique...The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both."-Heredity

From Reviews of the Second Edition...

"...highly recommended for those researchers who use in situ hybridization in their work or for those who want to exploit for the first time the possibilities that this technique offers biomedical research" -European Urology Today

"...should be available in every major biomedical library as it represents the approach that morphological scientists can use to determine gene expression under different physiological and disease conditions in mature and developing tissues." -Journal of Anatomy

From the reviews of the third edition:

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